BR24992914 "Integrated biotechnological solutions for production of value added products aimed at increasing the value of lignocellulosic by-products"

Within the framework of the development of highly effective and ecological methods of preventive maintenance of large-scale lignocellulose secondary products, oriented to the production of products with added cost:

- Optimal parameters of the technological regime for the transformation of organic substances, including the production of sugar for molecular water, have been established;

- Improved technological scheme for obtaining molecular water from lignocellulose secondary cheese;

- We have improved the method of changing organic things, including the number of Sakharov.

1) the main supply is concluded in low-concentration low-temperature hydrolysis, increased mechanical breakdown (centrifugation 7500g, 15min), filtration and controlled dilution of hydrolysates (0-, 2-, 5-, 10-cratnoe) with subsequent sterilization (120-121 °C, 20 min).

2) developed modes of hydrolysis allow to ensure selective dilution of hemicellulose and specific destruction of lignin at the disposal of cellulose, which has been analyzed by hydrolysers.

3) eggshells and fruit crops demonstrate more high availability of reducing Sakharov.

Bayadilova A. A., Abdilova G. B., Bekbayev K. S., Toleugazykyzy A., Amirkhanov Sh.A. methods of extraction of cent components from vinogradny vzhimok. Vestnik University Shakarima. Series of technical sciences. 2025;(2(18)):177-185.

https://doi.org/10.53360/2788-7995-2025-2(18)-21,

Published: June 27, 2025

https://drive.google.com/file/d/1rkhL_BJ_Aq89Km83ZoR1RZbI0tbA0UO9/view?usp=drive_link] according to the results of coffee withdrawal, the amount of phenolic inhibitors

[https://doi.org/10.1016/j.indcrop.2025.122068]. dilution 2-5-crater effectively neutralizes the initial sensitivity (pH > 5.5), predetermined inhibition of microbial producers (E. coli, Clostridium spp., S. cerevisiae).

Effective conditions and parameters for preliminary procurement and fermentation of large-scale lignocellulose products and their production and demand for their structural characteristics and potential for production of products were established increased cost (enzymatic Sakharov, biovodoroda, biomethana, bioethanola, biobutanola, biomass, etc.) D.) with the use of various types and strains of microorganisms on the basis of the analysis of the formation of permafrost and concomitant products (mixed with Sakharov, furanovy and phenolic compounds, organic substances, chemical needs in the environment, public and lethargic other substances and Dr.) at the stages of hydrolysis and anaerobic absorption.

1) based on the results of research on microbial synthesis in the biovodoro from rapeseed Solom with the use of Escherichia coli strain BW25113, optimal parameters were established in the process [https://drive.google.com/file/d/1wsX1A7mOt0kCIlG_T2XC5afYTRJ6oRKj/view?usp=drive_link].

1) on the basis of the received results developed technological instructions-standard of the organization of NAO "Shakarim University" on biovodorod, received a method of dark fermentation of substrates on the basis of Pshenichny (St NAO 130840007973-1-10-2025) [https://drive.google.com/file/d/1kpqu3j8mN4yeFpmGf2VKrQRpm6HYRULk/view?usp=drive_link I rapeseed Solom( St NAO 130840007973-2-10-2025) [https://drive.google.com/file/d/1MRaatz5yYyEqYXhE-kiL7inl8bGEk4Kd/view?usp=drive_link with the use of water-producing bacteria, prescribed for the introduction of the production in the quality of the upper Gaza (E949).

Seven articles published in peer-reviewed scientific journals, indexed in the databases of the web of Science and Scopus:

1. Vanyan L., Toleugazykyzy A., Yegizbay K., Daniyarova A., Zuloyan L., Mikoyan G., Vassilian A., Poladyan A., Bekbayev K., Trchounian K. Microbial Valorization of Sunflower Husk for Sustainable Biohydrogen and Biomass Production // Energies. – 2025. - Vol. 18. - No. 14. – P. 3885. - https://doi.org/10.3390/en18143885 percentile-64, Q3

Published: July, 2025

2. Avetisyan R., Petrosyan H., Toleugazykyzy A., Bolkenov B., Bekbayeva R., Bekbayev K., Trchounian K., Poladyan A. Conversion of spent coffee grounds into bioenergy and biomass using Clostridium species: A sustainable pathway for renewable energy // Industrial Crops & Products. – 2025. – Vol. 236th-Article 122068 – - https://doi.org/10.1016/j.indcrop.2025.122068 percentile-92, Q1

Published: October 11, 2025

3. Toleugazykyzy A., Bekbayev K., Bolkenov B., Alwazeer D., Rskeldiyev B., Kuterbekov K., Bekmyrza K., Kabyshev A., Kubenova M., Opakhai S. Comprehensive Review of Recent Trends in the Use of Deep Eutectic Solvents for the Valorization of Secondary Lignocellulosic Biomass // Sustainability - 2025, 17(21), 9492; https://doi.org/10.3390/su17219492

Percentile-86, Q2

Published: October 24, 2025

4. Trchounian K., Shirvanyan A., Daniyarova A., Vassilian A., Poladayan A., Kumar G., Orynbekov D., Bekbayev K. Biomass and bioethanol production from pretreated mixed fruit peel hydrolysate using Saccharomyces cerevisiae strains at different pH and oxygen conditions. BMC Biotechnol 25, 136 (2025). https://doi.org/10.1186/s12896-025-01074-1

Percentile - 65, Q2

Published: 09 December 2025

5. Karaogul E., Al Çiçek S., Uğurtay A., Engin T., Çiğdem A., Alwazeer D., Bayadilova A., Bekbayeva R., Orynbekov D., Bekbayev K. Optimizing supercritical CO₂ extraction of phytochemicals from grape peels using response surface technology: Modeling, characterization, and antioxidant potential. Applied Food Research 6(1), 101579 (2026).

https://doi.org/10.1016/j.afres.2025.101579

Percentile - 90, Q1

Published: 10 December 2025

6. Engin T., Karaogul E., Al Çiçek S., Çiğdem A., Alwazeer D., Bekbayev K., Abdilova G., Bolkenov B. Recovery of phytochemicals from cimin black grape seeds with sub/super-critical carbon dioxide extraction. The Journal of Supercritical Fluids 232, 106894 (2026).

https://doi.org/10.1016/j.supflu.2026.106894

Percentile - 92, Q1

Published: 14 January 2026

7. Alwazeer D., Karaogul E., Al Çiçek S., Ugurtay A., Çiğdem A., Engin T., Bekbayev K., Abdilova G., Bolkenov B., Orynbekov D. A Novel Hydrogen-incorporated Carbon Dioxide Supercritical System for Extracting Phytochemicals from Grape Peels: Response Surface Methodology–Based Modeling and Optimization. Results in Engineering 109142 (2026).

https://doi.org/10.1016/j.rineng.2026.109142

Percentile - 75, Q2

Published: 14 January 2026

Six articles published in peer-reviewed domestic scientific journals, included in the list of recommended articles:

1.Tlevlesova D. A., Bolkenov B. T., Bekbaeva R. S., Bekbaeva K. S., Daniyarova A. E. introduction of preliminary preparation of Pivot droppings on the parameters of fermentation and Water Products // Chemical Technologies. – 2025. – № 1 (162). – Pp. 116-127. – https://doi.org/10.55956/VMBN7659

Published: September 30, 2025

2. Bayadilova A. A., Abdilova G. B., Bekbayev K. S., Toleugazykyzy A., Amirkhanov Sh.A. methods of extraction of valuable components from grape extract // Vestnik University Shakarima. Series of technical sciences. – 2025. – № 2 (18). - Pp. 177-185. – https://doi.org/10.53360/2788-7995-2025-2(18)-21

Published: June 27, 2025

3. Тлевлесова Д.А., Болкенов Б.Т., Бекбаева Р.С., Бекбаев К.С., Даниярова А.Е. Влияние предварительной обработки пивной дробины на параметры ферментации и продукцию водорода // Химические технологии. – 2025. – № 1 (162). – С. 116–127. – https://doi.org/10.55956/VMBN7659

Published: September 30, 2025.

4. Ергазиева Г.Е., Мамбетова М.М., Бекбаев К.С., Төлеуғазықызы А., Даниярова А.Е. Анализ текущего состояния и технологий конверсии лигноцеллюлозных материалов // Институт проблем горения. Горение и плазмохимия. – 2024. – Т. 22. – № 4. – С. 343–362. – https://doi.org/10.18321/cpc22(4)343-362

Published: December 24, 2024.

5. A.A.Melissova, A.D.Daiyrbekova, M.Zh. Nurbekova, A.R. Okassov, B.T. Bolkenov, K.S. Bekbayev, Application of molecular hydrogen in food production and preservation: curren trends and future prospects (a review) // Vestnik KazUTB https://doi.org/10.58805/kazutb.v.4.29-1200

Published: December 29, 2025.

6. A.E. Daniyarova, K.S. Bekbayev, A. Toleugazykyzy, Zh. Kalibekkyzy, K.P. Moldakashev, Inhibitors of lignocellulosic biomass: mechanisms of action and impact on the efficiency of biological hydrogen production (review) // Вестник Университета Шакарима. Технические науки. – 2025. – № 4(20). – С. 455–466. – DOI: 10.53360/2788-7995-2025-4(20)-54.

Published: November 14, 2025.

Two theses were published in the materials of the international scientific conference.

1. Mikoyan G., Vanyan L., Baichiyeva K., Yegizbay K., Bekbayev K., Trchounian K. Growth and Hydrogen Production of Escherichia Coli BW25113 in Mixtures of Sugar Beet Pulp and Sugar Beet Molasses // Journal of the Institute of Science and Engineering for Environmental Solutions (JISEES). – 2025. – Vol. 1. – Special Issue 1. – P. 68.

https://doi.org/10.46991/JISEES.2025.SI1.068 Published: October 21, 2025

2. Toleugazykyzy A., Daniyarova A., Bekbayev K., Xu M., Liu Y., Liao W. Sugar Beet Residue Valorization Through Anaerobic Digestion for Hydrogen and Methane Generation // Journal of the Institute of Science and Engineering for Environmental Solutions (JISEES). – 2025. – Vol. 1. – Special Issue 1. – P. 162. https://doi.org/10.46991/JISEES.2025.SI1.162 Published: October 21, 2025

Two patents on field models received:

1. Bekbayev K. S., Toleugazykyzy A., Daniyarova A. E., Bekbaeva R. S., Yesengaliuly N. Sposob fermentation of organic material with the receipt of biogas / / patent Republic of Kazakhstan on polesical model No. 11141 https://drive.google.com/file/d/1rkhL_BJ_Aq89Km83ZoR1RZbI0tbA0UO9/view?usp=drive_link

2. Bekbayev K. S., Toleugazykyzy A., Bolkenov B. T., Bekbaeva R. S., Bayadilova A. A. Thanks to the receipt of an extract from a rare cheese / / patent of the Republic of Kazakhstan on the polesical model No. 11184 https://drive.google.com/file/d/1rkhL_BJ_Aq89Km83ZoR1RZbI0tbA0UO9/view?usp=drive_link

Development of 2 standards of the organization of esperiments:

1. Biovodorod from substrate on the basis of simple Solom St NAO 130840007973-1-10-2025, [https://drive.google.com/file/d/1kpqu3j8mN4yeFpmGf2VKrQRpm6HYRULk/view?usp=drive_link]

2. Biovodorod of substrate on the basis of solomys rapeseed (St NAO 130840007973-2-10-2025) [https://drive.google.com/file/d/1MRaatz5yYyEqYXhE-kiL7inl8bGEk4Kd/view?usp=drive_link]

5 (five) master's theses prepared and protected in 2025 within the framework of NTP:

1. Bolgas Nude Berikovna:

* Kazakh agrotechnical research university named after S. Seifullin, 7m07201-Food Technology,

https://drive.google.com/file/d/1J7YVA3Ut0JsREsuoNyC0OnCRPJa4h_mc/view?usp=drive_link

• Northwest A&F University, Biology and Medicine Food Engineering, https://drive.google.com/file/d/1Iw_SoWtCxohO5nS3xZGtGAb1pmAznJ-0/view?usp=drive_link

2. Mendybayeva Ayazhan nurlanovna-Kazakh agrotechnical research university named after S. Seifullin, 7m07201-Food Technology,

https://drive.google.com/file/d/14PbeQvnAVSXocCiZ02QccB4TstEg1UrZ/view?usp=drive_link

3. Zhumagalieva Ainazhan Kairbolatovna-Kazakh agrotechnical research university named after S. Seifullin, 7m07201-Food Technology,

https://drive.google.com/file/d/1BorDcfgPbTZJjiiSAkyMQVPPh146Ni3_/view?usp=drive_link

4. Kabylbek Akbope Alimbekovna-Al-Farabi Kazakh National University, 7m01503 chemistry, https://drive.google.com/file/d/1UrIZXqOhLdQqzQrwGNSYTAKBHy6uc0PX/view?usp=drive_link

5. Zhuginis Bekmukhamet Abylkasymovich-Al-Farabi Kazakh National University, 7m01503 chemistry,

https://drive.google.com/file/d/1F_6hJWKJ_2BgDVTNJ_q2fl08xhD45C3q/view?usp=drive_link