AP23488216 "Development of technology for chemical modification of chlorella cultivation using biopolymers and nanoparticles to create biologically active substances"
SDG #12
Project Leader
Kassymova Zhanar Sailaubekovna, Candidate of Biological Sciences, Associate Professor
Relevance
The idea of the project is to develop an innovative technology for chemical modification of nutrient media using biopolymers (chitosan, sodium carboxymethylcellulose) and nanoparticles (iron, silver), which allows increasing biomass productivity in laboratory and production conditions. The project is dedicated to the development of an environmentally friendly, low-cost, resource-saving feed additive for farm animals and birds, as well as a biofertilizer for soil and a viability stimulator for plant seeds with biological activity.
Goal
The goal of the project is to develop a technology for chemical modification of the cultivation of chlorella microalgae using biopolymers and nanoparticles to obtain biologically active substances (BAS) as feed additives, biofertilizers and biostimulators of growth and development.
Expected Result
1) The physico-chemical modification of chlorella cultivation in a closed-type photobioreactor will be carried out;
2) A study of chemical modification of chlorella cultivation using nanoparticles (silver and iron) and biopolymers (chitosan and sodium carboxymethylcellulose) and biochemical analysis for biologically active substances will be performed;
3) The effect of biologically active substances of chlorella on soil fertility and viability of vegetable seeds in laboratory experiments will be studied;
4) The technique of complex tillage of soil and plant seeds will be worked out in the conditions of agricultural production of Prirechnoye Agrofirms LLP;
5) Modification of the manufactured forms of chlorella preparation (granules, tablets, suspension, paste) will be carried out;
6) Pilot tests will be conducted on the use of various doses and forms of biological active additive feed in diets to study the effect of chemical modification of cultured chlorella on the productivity and immunological status of animals and birds;
7) Instructions for the use of the developed technology will be developed;
8) 1 doctoral dissertation will be prepared, 2 (two) articles will be published in scientific publications indexed in the Science Citation Index Expanded and included in the 1st (first) and (or) 2nd (second) quartile by impact factor in the Web of Science database and (or) having a CiteScore percentile in the Scopus database of at least 65 (sixty-five), and a 1 (one) article in domestic publication recommended by CCSES.
Achieved Result
- The physico-chemical modification of chlorella cultivation in a closed-type photobioreactor was carried out.
Physico-chemical modification of chlorella cultivation was carried out in order to obtain chlorella protein products (selection of nutrient media, temperature, lighting, pH) and biochemical analysis for biologically active substances.
The development of a closed-type photobioreactor model with optimal physico-chemical parameters of cultivation was carried out.
- A study of chemical modification of chlorella cultivation using nanoparticles (silver and iron) and biopolymers (chitosan and sodium carboxymethylcellulose) and biochemical analysis for biologically active substances was performed.
Synthesis of silver and iron nanoparticles was carried out by chemical reduction of metal cations in solutions with environmental and non-toxic reducing agents and stabilizers (glucose, ascorbic acid, chitosan, carboxymethylcellulose).
Chemical modification of chlorella cultivation using nanoparticles (silver and iron) and biochemical analysis for biologically active substances was performed.
Chemical modification of chlorella cultivation using biopolymers (chitosan and sodium carboxymethylcellulose) and biochemical analysis for biologically active substances was performed.
- The effect of biologically active substances of сhlorella on soil fertility and viability of vegetable seeds in laboratory and field experiments has been studied. Vegetation field experiments were conducted to study the effect of chlorella suspension as a biostimulator on the viability of corn seeds. The effect of chlorella suspension on soil resistance to wind and water erosion and agrochemical parameters of soil fertility was studied in model laboratory experiments.
- Vegetative laboratory experiments were conducted to study the effect of chlorella suspension as a biostimulator on the viability of seeds (germination, fertility and germination energy).
- A laboratory experiment was conducted to study the effect of chlorella biofertilizer on erosion resistance and agrochemical parameters of the soil.
- The technique of complex tillage of soil and plant seeds in the conditions of agricultural production of Agrofirmy Prirechnoye LLP has been worked out.
- 1 (one) article was published in a domestic publication recommended by the CCSES (Orazzhanova L.K., Kasymova Zh.S., Nurtazina Zh.Zh., Aserzhanov D.K., Kabdulkarimova K.K. Synthesis and application of iron nanoparticles for the production of biologically active substances Chlorella vulgaris // Reports of the NGO "National Academy of the Republic of Kazakhstan". – 2025. – № 354 (2). – Pp. 275-290. https://doi.org/10.32014/2025.2518-1483.358,
Published: June 20, 2025
1 (one) article has been published in a foreign journal with a 91 percentile CiteScore in the Scopus database
- (Z. Kassymova, L.Orazhanova, D. Asserzhanov, Z. Nurtazina, A. Sapakova, A. Sabitova, N. Nurgaliev. Synthesis of Silver Nanoparticles Using Biopolymers for Cultivation of Chlorella vulgaris Protein Biomass // ES Materials and Manufacturing. – 2025. – 28, 1576. https://dx.doi.org/10.30919/mm1576 , the percentile is 91.
Published on June 14, 2025
1 (one) article was published in the collection of scientific papers
- (Kiarstanova N.B., Beisenova D.D., Kasymova Zh.S., Orazzhanova L.K. Chlorella vulgaris microbaldyryn onimdiligin arttyru ushin temir nanobolshekterin alu zhane koldan // Proceedings of the Republican scientific and practical conference of young scientists and students "XXI century: science and innovation". – Taldykorgan, I. Zhansugurov Zhetysu University, 2025, pp. 184-188. https://zhetysu.edu.kz/wp-content/uploads/2025/04/%D0%A1%D0%B1%D0%BE%D1%80%D0%BD%D0%B8%D0%BA-%D0%BA%D0%BE%D0%BD%D1%84%D0%B5%D1%80%D0%B5%D0%BD%D1%86%D0%B8%D0%B8-07.04.25.pdf,
Published on April 28, 2025
The effect of biologically active substances of chlorella on soil fertility and viability of vegetable seeds in laboratory and field experiments has been studied. Vegetation field experiments were conducted to study the effect of chlorella suspension as a biostimulator on the viability of corn seeds. The effect of chlorella suspension on soil resistance to wind and water erosion and agrochemical parameters of soil fertility was studied in model laboratory experiments.
A laboratory experiment was conducted to study the effect of chlorella biofertilizer on erosion resistance and agrochemical parameters of the soil.
1 (one) article was published in a domestic publication recommended by the KOKNVO (Orazzhanova L.K., Kasymova Zh.S., Nurtazina Zh.Zh., Aserzhanov D.K., Kabdulkarimova K.K. Synthesis and application of iron nanoparticles for the production of biologically active substances Chlorella vulgaris // Reports of the NGO "National Academy of the Republic of Kazakhstan". – 2025. – № 354 (2). – Pp. 275-290. https://doi.org/10.32014/2025.2518-1483.358,
Published: June 20, 2025
1 (one) article has been published in a foreign journal with a 91 percentile CiteScore in the Scopus database (Z. Kassymova, L.Orazhanova, D. Asserzhanov, Z. Nurtazina, A. Sapakova, A. Sabitova, N. Nurgaliev. Synthesis of Silver Nanoparticles Using Biopolymers for Cultivation of Chlorella vulgaris Protein Biomass // ES Materials and Manufacturing. – 2025. – 28, 1576. https://dx.doi.org/10.30919/mm1576 , the percentile is 91.
Published on June 14, 2025
1 (one) article was published in the collection of scientific papers (Kiarstanova N.B., Beisenova D.D., Kasymova Zh.S., Orazzhanova L.K. Chlorella vulgaris microbaldyryn onimdiligin arttyru ushin temir nanobolshekterin alu zhane koldan // Proceedings of the Republican scientific and practical conference of young scientists and students "XXI century: science and innovation". – Taldykorgan, I. Zhansugurov Zhetysu University, 2025, pp. 184-188. https://zhetysu.edu.kz/wp-content/uploads/2025/04/%D0%A1%D0%B1%D0%BE%D1%80%D0%BD%D0%B8%D0%BA-%D0%BA%D0%BE%D0%BD%D1%84%D0%B5%D1%80%D0%B5%D0%BD%D1%86%D0%B8%D0%B8-07.04.25.pdf,
Published on April 28, 2025
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