AP26198315 «Scientific and practical aspects of technology development of functional dairy products based on new generation biologically active additives».

- Based on theoretical research, an analysis of scientific literature was conducted to identify the main characteristics of probiotic microorganism strains capable of producing postbiotics with antioxidant, anti-inflammatory, and immunomodulatory properties.

It was established that rod-shaped lactic acid bacteria (Lactiplantibacillus, Lacticaseibacillus, Levilactobacillus) are producers of antioxidant peptides, enzymes, and bacteriocins; coccoid lactic acid bacteria (Lactococcus, Leuconostoc, Weissella) produce immunomodulatory and techno-functional exopolysaccharides; and yeasts of the genus Saccharomyces are sources of β-glucans, cell wall mannans, mannoproteins, peptides, and amino acids with pronounced anti-inflammatory and immunomodulatory effects.

- Based on the conducted experimental studies, the identification and selection of the most promising probiotic microorganism strains with high potential for the production of postbiotics possessing antioxidant, anti-inflammatory, and immunomodulatory activity were carried out.

As a result of morphological and tinctorial screening of cultures isolated from traditional fermented products (kumis, shubat, sauerkraut), strains of microorganisms were identified, including rod-shaped and coccoid lactic acid bacteria (Lacticaseibacillus, Leuconostoc) and yeasts of the genus Saccharomyces. It was established that yeast isolates from kumis are characterized by the potential to synthesize β-glucans and mannans; lactic acid bacteria from shubat demonstrate the ability to produce peptides, organic acids, and bacteriocins; and sauerkraut isolates produce exopolysaccharides.

The obtained results confirm the prospects of the selected strains as producers of targeted postbiotic fractions and provide a basis for further research on postbiotic production.

English:

Based on the research results, an article was published in the SHEQAC of MSHE of RK

Zharykbasov E. S., Kakimova Zh. Kh., Zharykbasova K. S., Kakimov A. K., Bepeeva A. E. Comparative analysis of postbiotic isolation methods and determination of preparation yields from microbial strains // Bulletin of Shakarim University, series “Technical Sciences”. – 2025. – No. 4 (20). – pp. 448–455.

https://doi.org/10.53360/2788-7995-2025-4(20)-53

Published: December 26, 2025.

Published: September 11, 2025.

- Based on experimental studies, the optimal cultivation conditions for the selected strains were determined, including temperature, pH, aeration, and nutrient medium composition parameters that ensure a consistently high yield of postbiotics under laboratory conditions. The stability and resistance of the microorganism strains were assessed by analyzing biomass growth depending on cultivation conditions.

It was established that cultivation of Lactobacillus and Leuconostoc strains on MRS medium at pH 6.1 ± 0.1, temperature 28–32 °C, under microaerophilic conditions resulted in the highest biomass increase (2.6–2.7 mg/cm³). For yeasts of the genus Saccharomyces, the optimal cultivation conditions were Sabouraud medium at pH 4.5 ± 0.5, temperature 30–32 °C, with mild aeration and moderate oxygen supply without turbulent mixing, under which biomass growth reached 3.2–3.5 mg/cm³.

Optical density analysis (OD₆₀₀) confirmed the reproducibility of the data: for Lactobacillus and Leuconostoc, OD₆₀₀ values consistently ranged from 6.0 to 7.8 units, while for Saccharomyces they ranged from 6.4 to 7.0 units. OD fluctuations in repeated experiments did not exceed ±5%, indicating the stability of the physiological state of the cultures and the consistent progression of metabolic processes.

Based on the research results, the following articles were published:

Bepeeva A. E., Zharykbasova K. S., Mirasheva G. O., Toleubekova S. S.

Postbiotics in food technologies // Innovative Development of the Food, Light Industry and Hospitality Industry: Proceedings of the International Practical Conference. — Almaty, 2025. — pp. 92–93.

Published: October 16, 2025.

Kakimova Zh. Kh., Zharykbasova K. S.

Optimization of cultivation conditions of probiotic strains for postbiotic production // Problems of Scientific and Practical Activity. Search and Selection of Innovative Solutions: Proceedings of the International Scientific and Practical Conference. — Samara, 2025. — pp. 57–61.

Published: November 13, 2025.

- Based on theoretical studies, an analysis of scientific literature, patent sources, and existing technologies was conducted to identify the most effective methods for isolating postbiotics from probiotic microorganism strains. By identifying the key factors influencing postbiotic yield and activity, three methods were selected: thermal treatment, ethanol precipitation, and ultrafiltration, as they combine technological simplicity, economic feasibility, and the ability to preserve biologically active compounds. This makes them the most reproducible and promising among existing approaches. A review of the literature confirmed the appropriateness of selecting these methods.

- Based on experimental studies and a comparative analysis of characteristics, the thermal method was selected as the most effective approach for obtaining postbiotics from probiotic microorganism strains, ensuring high purity, concentration, and preservation of biological activity. The thermal method allows achieving the highest dry matter yield—on average 90–92%—high antioxidant activity (86–89%), and complete microbiological safety, regardless of the strain type.

Thus, as a result of the research, an optimal method for isolating postbiotics from probiotic strains was identified, combining technological simplicity, reproducibility, and preservation of the functional properties of biologically active compounds.

-- The percentage yield of postbiotics relative to the total biomass of microorganisms was determined using the thermal isolation method, which ensures a high degree of purification and preserves the biological activity of the postbiotics. It was established that the postbiotic yield for Lacticaseibacillus sp. was 88.0 ± 3.0%, for Leuconostoc sp. — 92.0 ± 2.0%, and for Saccharomyces sp. — 85.0 ± 3.0%.

As a result of this research, the following article was published in the SHEQAC journal:

Kakimova Zh. Kh., Zharykbasova K. S., Bepeeva A. E., Mirasheva G. O., Beisembaева G. Sh.

Postbiotic Producers for Functional Dairy Products // Bulletin of Shakarim University. Series “Technical Sciences.” — 2025. — № 3(19). — P. 463–472.

Multidisciplinary direction

https://doi.org/10.53360/2788-7995-2025-3(19)-52

Published: September 30, 2025.

Additionally, an article has been submitted to the SHEQAC journal:

"Comparative Analysis of Postbiotic Isolation Methods and Determination of Product Yields from Microbial Strains" (Zharykbasov E. S., Kakimova Zh. Kh., Zharykbasova K. S., Kakimov A. K., Bepeeva A. E.), accepted for publication in Bulletin of Shakarim University, Series “Technical Sciences” No. 4 (20), 2025.

Publication acceptance certificate provided.